02052naa a2200325 a 450000100080000000500110000800800410001902400420006010000160010224501170011826000090023552010820024465000190132665000160134565000110136165000350137265000240140765300200143165300250145165300260147665300400150270000140154270000180155670000150157470000200158970000160160970000140162570000170163977300700165610026992020-01-17       2007    bl uuuu     u00u1 u    #d7 a10.1016/j.molbiopara.2007.04.0032DOI1 aSIMÕES, M.  aSingle nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni.h[electronic resource]  c2007  aSingle nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope  aBioinformatics  aCathepsin B  aModels  aSingle nucleotide polymorphism  aSchistosoma Mansoni  aBioinformática  aComparative modeling  aComputational biology  aPolimorfismo de nucleotídeo único1 aBAHIA, D.1 aZERLOTINI, A.1 aTORRES, K.1 aARTIGUENAVE, F.1 aNESHICH, G.1 aKUSER, P.1 aOLIVEIRA, G.  tMolecular and Biochemical Parasitologygv. 154, p. 134-140, 2007.