02074naa a2200253 a 450000100080000000500110000800800410001902400300006010000160009024501280010626000090023452012770024365000220152065300460154265300110158865300250159970000200162470000210164470000210166570000260168670000220171270000190173477300670175312562532023-03-15       2009    bl uuuu     u00u1 u    #d7 a10.4238/vol8-1gmr5772DOI1 aMÉO, S. C.  aCharacterization of mitochondrial genotypes in the foundation herd of the Canchim beef cattle breed.h[electronic resource]  c2009  aThe Canchim (5/8 Charolais + 3/8 Zebu) beef cattle breed was developed at Southeast-Embrapa Cattle to take advantage of hybrid vigor and to combine the higher growth rate and beef quality of Charolais with tropical adaptations of Zebu. The development of three lineages (old, new, and crossbred) has increased its genetic basis. The genotypic origin (Bos taurus or Bos indicus) of the mitochondrial DNA (mtDNA) of the Canchim breed was unknown. We characterized the mtDNA genotype of this founder herd by allele-specific polymerase chain reaction. The 173 founder Zebu females (62 Indubrasil, 3 Guzerat, and 108 Nellore) and their 6749 offspring were identified. The frequency of B. indicus mtDNA ranged from 1.15 to 2.05% among the descendants (n = 6404) of each maternal line with available DNA, and among animals that were alive (n = 689) in December 2007 among the three lineages. Though mtDNA characterization can be used to direct animal selection, the low frequency of B. indicus mtDNA impairs the evaluation of its effects on production traits in these animals. The high prevalence of B. taurus mtDNA in Canchim proves that the founder Zebu females from the Indubrasil, Guzerat and Nellore breeds were obtained from crosses of Zebu sires with local B. taurus dams.  amitochondrial DNA  aAllele specific polymerase chain reaction  aBovine  aMaternal inheritance1 aFERREIRA, C. R.1 aCHIARATTI, M. R.1 aMEIRELLES, F. V.1 aREGITANO, L. C. de A.1 aALENCAR, M. M. de1 aBARBOSA, P. F.  tGenetics and Molecular Researchgv. 8, n. 1, p. 261-267, 2009.