01704naa a2200241 a 450000100080000000500110000800800410001910000180006024500810007826000090015952010290016865000160119765000210121365000180123465000130125265300120126565300250127765300150130265300180131770000170133570000200135277300900137212126662004-11-04       2003    bl uuuu     u00u1 u    #d1 aGUERRA, M. P.  aEmbryo culture and in vitro multiplication of Prunus 'Capdeboscq' rootstock.  c2003  aEmbryo culture and recovery are techniques used in basic and applied studies of morphogenesis, in vitro propagation and genetic in the Prunus genus. The present study assessed the germination and organogenetic development of embryos in culture media with different concentrations of GA3 as well as the in vitro multiplication rate of clones of the 'Capdeboscq' rootstock. Embryonic axes were excised and inoculated in Lepoivre culture medium supplemented with BAP (0.1 mg L-1), NAA (0.1 mg L-1) and GA3 (0.0; 1.0; 2.5; 5.0;7.5 and 10.0 mg L-1). The in vitro multiplication  potential of four clones was assessed after embryo germination using the double phase multiplication method in the last subculture. The results showed a mean of 70.7% in vitro germination. GA3 was not effective in the germination, but induced greater elongation of the embryo and inhibited the root development. The clones obtained in the embryo culture showed high potential for in vitro multiplication with rates of 6.9 and 14.9 shoots per explant.  agermination  agibberellic acid  aorganogenesis  aEmbrião  aCultura  aIn vitro propagation  aPeach tree  aPorta-enxerto1 aROGALSKI, M.1 aSILVA, A. L. da  tCrop Breeding and Applied Biotechnology, Londrinagv. 3, n. 2, p. 141-147, June 2003.