02488naa a2200301 a 450000100080000000500110000800800410001902400520006010000200011224500890013226000090022152015910023065000240182165000130184565000210185865300190187965300190189865300240191765300210194165300290196265300140199170000210200570000160202670000230204270000230206570000180208877300800210621620612024-02-16 2023 bl uuuu u00u1 u #d7 ahttps://doi.org/10.1007/s11274-023-03622-32DOI1 aJESUS, G. L. de aNutrient uptake in supplemented substrate by oyster mushroom.h[electronic resource] c2023 aSpent mushroom substrate (SMS) is a promising alternative for supplementing oyster mushroom substrate, replacing conventional cereal bran. Therefore, the objective was to evaluate the production of Pleurotus ostreatus supplemented with Lentinula edodes’ SMS, through the nutritional analysis of the substrate. Wheat straw was used as substrate and supplemented with rice bran (RB) or SMS in 0%, 7%,15% and 30%. Ca, K, Mg, Mn, Zn, Cu and Fe contents of the cultivation substrates (before and after harvest) were determined through atomic absorption spectrophotometry. Mycelial growth (cm²/day), mycelial time colonization (days), number of clusters, number of pileus, average clusters weight (g), pileus lenght (cm) and width (cm), productivity (1st, 2nd and 3rd flush) (%), biological efficiency (%) of mushrooms were evaluated. Results indicated mycelial growth was higher (0.87 cm²/day compared to the Control) when the substrate was supplemented regardless of the source. The proportions of 15% of SMS achieved the highest biological efficiency (107% − 15% SMS versus 66% - Control). The only nutrients that showed differences in absorption were Ca, K and Mn, in which substrates supplemented with SMS had greater absorption of Ca (5.37 g.kg− 1 versus 1.94 g.kg− 1 in Control) while substrates supplemented with RB absorbed more K (6.56 g.kg− 1 versus 3.74 g.kg− 1 in Control). The mineral composi- tion of the substrate has a direct impact on the growth and yield of P. ostreatus, highlighting the potential of SMS as a alternative to traditional bran supplementation. aPleurotus ostreatus aCogumelo aLentinula Edodes aMacronutrients aMicronutrients aNutrient absorption aShimeji mushroom aSpent mushroom substrate aSubstrato1 aLAVORANTI, O. J.1 aSCHAFER, G.1 aOLIVEIRA, G. D. de1 aSILVA, R. S. de A.1 aCUQUEL, F. L. tWorld Journal of Microbiology and Biotechnologygv. 39, n. 7, p. 1-9, 2023.