02987naa a2200373 a 450000100080000000500110000800800410001902400520006010000260011224501500013826000090028852018050029765000180210265000140212065300360213465300260217065300150219665300260221165300290223765300400226665300270230670000240233370000230235770000200238070000180240070000220241870000140244070000210245470000210247570000200249670000290251670000140254577300540255921614362024-01-30       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s42860-023-00259-z2DOI1 aOLIVEIRA, K. C. B. F.  aDevelopment of a nanostructured film containing palygorskite and dermaseptin 01 peptide for biotechnological applications.h[electronic resource]  c2023  aClay minerals are suitable matrices to anchor organic molecules such as antimicrobial peptides (AMPs) so that their bioactivity is maintained, enabling the formation of new materials with potential for new applications in biotechnology. The objective of the present study was to develop a nanostructured flm where the properties of palygorskite (Plg) were combined at the molecular level with Dermaseptin 01 (DRS 01), in which the clay mineral also served as a substrate for the immobilization of this peptide. The flms were prepared using the Layer-byLayer (LbL) self-assembly technique. Crude palygorskite without purifcation (Plg IN) was subjected to physical and chemical procedures to increase its adsorptive properties. The structure, chemical composition, and morphology of Plg were investigated by X-ray difraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray fuorescence spectrometry (XRF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). LbL flms were adsorbed onto ITO (Indium Tin Oxide) and characterized electrochemically by cyclic voltammetry (CV), UV-Visible spectroscopy, and atomic force microscopy (AFM). For the ITO/DRS 01 and ITO/Plg/DRS 01 flms, an oxidation process at +0.77 V was observed, confrming that the DRS 01 maintained its electroactive behavior and intrinsic properties. The results also showed that Plg served as excellent support for the immobilization of DRS 01, increasing its concentration and availability in the flm form. This work reported immobilizing the DRS 01 peptide with Plg for the frst time in an ultrathin flm with bioactive properties. Thus, the flm developed can be explored for applications such as biosensor devices and antimicrobial coating materials as well as other biotechnological applications.  aBiotecnologia  aPeptídeo  aCationic antimicrobial peptides  aFibrous clay minerals  aFilme fino  aFilme nanoestruturado  aMineral argiloso fibroso  aPeptídeo antimicrobiano catiônico  aPithecopus hipocondria1 aFARIAS, E. A. de O.1 aTEIXEIRA, P. R. S.1 aBRANDÃO, V. S.1 aSÁBIO, R. M.1 aARAÚJO, A. R. de1 aEATON, P.1 aBERTOLINO, L. C.1 aBEMQUERER, M. P.1 aBARUD, H. da S.1 aLEITE, J. R. de S. de A.1 aEIRAS, C.  tClays and Clay Mineralsgv. 71, p. 600-615, 2023.