01549naa a2200469 a 450000100080000000500110000800800410001902200140006002400520007410000240012624501520015026000090030250000480031152002070035965000180056665000100058465000160059465000130061065300180062365300090064165300140065065300160066465300230068065300150070365300150071865300260073365300120075970000170077170000180078870000200080670000220082670000210084870000200086970000170088970000210090670000200092770000160094770000150096370000170097870000300099577300540102521598872024-02-06 2023 bl uuuu u00u1 u #d a2673-34397 ahttps://doi.org/10.3389/fgeed.2023.12410352DOI1 aHERNANDES-LOPES, J. aEnabling genome editing in tropical maize lines through an improved, morphogenic regulator-assisted transformation protocol.h[electronic resource] c2023 aNa publicação: Fernanda Rausch-Fernandes. aHere, we report the successful GE of agronomically relevant tropical maize lines using a MR-based, Agrobacterium-mediated transformation protocol previously optimized for the B104 temperate inbred line. aAgrobacterium aMilho aProtoplasto aZea Mays aAgrobactéria aB104 aBABY BOOM aCRISPR/Cas9 aEdição de genoma aOff-target aProtoplast aVIRESCENT YELLOW-LIKE aWUSCHEL1 aPINTO, M. S.1 aVIEIRA, L. R.1 aMONTEIRO, P. B.1 aGERASIMOVA, S. V.1 aNONATO, J. V. A.1 aBRUNO, M. H. F.1 aVIKHOREV, A.1 aFERNANDES, F. R.1 aGERHARDT, I. R.1 aPAUWELS, L.1 aARRUDA, P.1 aDANTE, R. A.1 aYASSITEPE, J. E. de C. T. tFrontiers in Genome Editinggv. 5, 1241035, 2023.