02669naa a2200445 a 450000100080000000500110000800800410001910000210006024501420008126000090022352013900023265000180162265000270164065000140166765000180168165000110169965000200171065000140173065000340174465000220177865000270180065000240182765300280185165300190187965300130189865300240191165300300193570000230196570000160198870000250200470000250202970000210205470000220207570000200209770000190211770000210213670000150215770000190217277300320219121596342023-12-13       2023    bl uuuu     u00u1 u    #d1 aTOLEDO, R. B. de  aEffect of food supplementation on in vitro embryo production and growth performance in prepubertal Nelore heifers.h[electronic resource]  c2023  aIn vitro embryos production from prepubertal heifers can help contribute to breeding programs; however, strategies are necessary to increase their embryo production. The aim of this study was to investigate the effects of two nutritional plans on oocyte recovery, embryo production and growth performance of prepubertal Nelore heifers. Thirty-four Nelore heifers with age of 6.5 months were divided into two feeding treatments (NP1 and NP2). The NP1 diets served as the control and NP2 diets were formulated to contain an average of 1.22-fold more energy than NP1. After 3 months of supplementation, the animals underwent follicular aspiration (ovum pick-up, OPU) every 21 d for 3 months and embryos were produced in vitro. Wither height, chest depth, body weight and subcutaneous fat of animals were measured. The number of retrieved and viable oocytes per OPU were 1.49-fold and 1.42-fold greater in NP2 heifers (p = 0.018 and p = 0.049, respectively) than those in NP1 heifers. Heifers administered NP2 produced 29.7% blastocysts, a percentage higher than NP1 animals that produced 24.40% embryos (p &lt 0.05). Consequently, females in the NP2 treatment showed improved body development. These results indicate a positive effect of a higher energy diet on assisted reproduction and body development in prepubertal heifers.  aBiotechnology  aIn vitro fertilization  aNutrition  aBiotecnologia  aBovino  aEmbrião Animal  aGado Zebu  aMelhoramento Genético Animal  aNutrição Animal  aPrograma de Nutrição  aReprodução Animal  aFertilização in vitro  aNovilha Nelore  aOócitos  aProdução in vidro  aSuplementação alimentar1 aFARIA, O. A. C. de1 aLEME, L. O.1 aMAGNABOSCO, C. de U.1 aGUIMARAES JUNIOR, R.1 aEIFERT, E. da C.1 aSANTOS, I. R. dos1 aOLIVEIRA, R. V.1 aDODE, M. A. N.1 aMALAQUIAS, J. V.1 aPIVATO, I.1 aMARTINS, C. F.  tAnimal Biotechnology, 2023.