01469nam a2200337 a 450000100080000000500110000800800410001910000270006024501720008726001280025930000110038752003870039865000240078565000130080965000160082265000350083865000090087365300160088265300170089865300320091565300100094765300120095770000190096970000210098870000200100970000200102970000210104970000190107070000210108970000210111021584822023-11-17       2023    bl uuuu     u00u1 u    #d1 aBEZERRA L. P. de A. F.  aEngenharia genética de precisão para tolerância à seca em soja e seu efeito na via de morte celular programada do retículo endoplasmático.h[electronic resource]  aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais... Florianópolis: SBGc2023  ap. 85.  aIn this study, we applied two strategies to suppress senescence induced by the DCD-NRP circuit to increase drought tolerance. Firstly, we investigated the potential of CRISPR/dCas9 system fused with transcription activators (VP64) in positively modulating the expression of GmBiP. For this, the efficiency of three single guide was tested in transient tobacco transformation assays.  aGenetic improvement  aSoybeans  aGlycine Max  aMelhoramento Genético Vegetal  aSoja  aCRISPR/Cas9  aCRISPR/dCas9  aCulturas tolerantes à seca  aGmBiP  aGmNAC301 aMELO, B. P. de1 aARRAES, F. B. M.1 aMORGANTE, C. V.1 aLOURENCO, I. T.1 aDOMICIANO, G. P.1 aANDRADE, R. V.1 aFONTES, E. P. B.1 aSÁ, M. F. G. de