02371naa a2200349 a 450000100080000000500110000800800410001902400550006010000190011524501290013426000090026352013070027265000140157965000110159365000180160465000240162265300320164665300220167865300350170065300220173570000260175770000200178370000190180370000190182270000250184170000180186670000190188470000230190370000200192670000200194677300550196621576032023-10-31       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1590/1984-3143-AR2023-01002DOI1 aDIAS, H. R. S.  aReproductive development of dairy heifers in an integrated livestock-forest system during the summer.h[electronic resource]  c2023  aThis study aimed to assess the cortisol, body and reproductive development of prepubertal Holstein and Holstein-Gir ¾ heifers at 27 months of age maintained in an integrated livestock-forest (ILF) system for 60 summer days compared to the monoculture system in full sun (FS). The ILF system promoted changes (P=0.02) in the cortisol levels of Holstein-Gir ¾ heifers and did not affect weight gain in any of the breed groups studied. Animals in ILF system presented a lower (P=0.006) vulvar development for the rima height parameter and similar for the vulva width parameter. The ovarian follicular population of Holstein-Gir ¾ heifers in the ILF system was lower (P=0.004); however, for the Holstein heifers, no statistical difference was found, and numbers were higher (P=0.08) in the ILF system. None of the other ovarian parameters studied had any changes, and we also found important racial differences. Weight gain (P=0.003), vulvar development (P&lt0.001), and mean follicular size (P=0.008) were higher in the Holstein-Gir ¾ animals. Based on such results, the effect of the ILF system at 27 months of age on stress and reproductive parameters in the Holstein breed is considered positive, although negative effects have been detected on reproductive parameters in the Holstein-Gir ¾ breed.  aBem-Estar  aBovino  aGado Leiteiro  aReprodução Animal  aDesenvolvimento reprodutivo  aEstresse térmico  aIntegração pecuaria-floresta  aSistema integrado1 aCAMARGO, A. J. dos R.1 aOLIVEIRA, G. F.1 aMOURÃO, A. M.1 aSARAIVA, N. Z.1 aCAMARGO, L. S. de A.1 aMULLER, M. D.1 aMARTINS, C. E.1 aNOGUEIRA, L. A. G.1 aBRANDÃO, F. Z.1 aOLIVEIRA, C. S.  tAnimal Reproductiongv. 20, n. 3, e20230100, 2023.