02593naa a2200373 a 450000100080000000500110000800800410001902200140006002400520007410000180012624501540014426000090029852015160030765000100182365000250183365000180185865000140187665000240189065000120191465000090192665000140193565300150194965300150196465300300197970000230200970000210203270000130205370000210206670000200208770000150210770000160212270000200213877300610215821565062024-03-20       2023    bl uuuu     u00u1 u    #d  a2524-63727 ahttps://doi.org/10.1186/s40793-023-00524-72DOI1 aMENDES, L. W.  aImpact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.h[electronic resource]  c2023  aAbstract: Background - Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a fox-susceptible and fox-resistant common bean cultivar. -- Results: Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion.  aBeans  aRhizosphere bacteria  aTranscriptome  aBactéria  aControle Biológico  aFeijão  aRaiz  aRizosfera  aEndosphere  aMetagenome  aPlant-microbe interaction1 aRAAIJMAKERS, J. M.1 aHOLLANDER, M. de1 aSEPO, E.1 aEXPÓSITO, R. G.1 aCHIORATO, A. F.1 aMENDES, R.1 aTSAI, S. M.1 aCARRIÓN, V. J.  tEnvironmental Microbiomegv. 18, n. 1, article 68, 2023.