02696naa a2200241 a 450000100080000000500110000800800410001902400530006010000220011324500980013526000090023330000140024252018740025665300080213065300190213870000260215770000190218370000190220270000190222170000190224070000190225977301760227821532232024-01-15       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/978-3-031-15889-6_72DOI1 aCORREIA, B. S. B.  aThe Use of NMR Based Metabolomics to Discriminate Patients with Viral.h[electronic resource]  c2023  a129 - 174  aInfectious diseases are one of the most common conditions impacting global health, being a matter of concern for health agencies due to their contagious capacity and periodic outbreaks of new diseases, such as the global pandemic COVID-19. Viruses are among the main causes of this illness and it is defined as obligate intracellular parasites for their need to have a host cell to live and reproduce, since they won?t produce proteins and compete for nutrients and metabolites leading to the alteration of the host metabolome. The diagnosis of these viral infections can be done by detecting viral particles or components, isolating the virus in cell culture, or even by evaluating immune responses. In this context, metabolomics comes as a very useful tool that reflects all ?omics? techniques and best represents the phenotype. Since water-soluble metabolites and lipids are the major molecular constituents of human plasma, their abnormalities are commonly observed during disease, which contributes to the understanding of physiology and pathology. Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) are the most widely used techniques in metabolomics. NMR spectroscopy has emerged as a valuable application due to its ability to identify compounds with simple sample preparation, in addition, to being a non-destructive, highly reproducible, and quantitative technique (primary ratio method). These features make NMR a valuable tool that is frequently used in metabolomics analysis, and nowadays used in the diagnosis of viral diseases. Therefore, in this chapter, we will address a short integrative description of viral diseases and diagnostics, metabolomics, and NMR concepts. Furthermore, we will explore the advances in NMR-based metabolomics applied in medicine, and finally, the viral diseases discriminated by NMR-based metabolomics.  aNMR  aViral diseases1 aDALLE PIAGE, P. M. F.1 aALMEIDA, L. S.1 aRIBEIRO, G. H.1 aPEIXOTO, C. S.1 aCOLNAGO, L. A.1 aCARDOSO, D. R.  tIn: COVID-19 Metabolomics and Diagnosis: Chemical Science for Prevention and Understanding Outbreaks of Infectious Diseases. Cham: Springer International Publishing, 2023.