01770naa a2200241 a 450000100080000000500110000800800410001902200140006002400340007410000200010824501600012826000090028852009980029765000130129565000110130865300100131965300460132965300220137570000220139770000220141970000210144177300660146221514892023-05-04       2022    bl uuuu     u00u1 u    #d  a1664-462X7 a10.3389/fpls.2022.8141192DOI1 aHOFFMANN, L. V.  aAntibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease.h[electronic resource]  c2022  aThe cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.  aSerology  aVírus  aCLRDV  aEnzyme-linked immunosorbent assay (ELISA)  aIntermediary host1 aBRANQUINHO, A. A.1 aBARROSO, P. A. V.1 aVASLIN, M. F. S.  tFrontiers in Plant Sciencegv. 13, article 814119, 5 p. 2022.