02451naa a2200349 a 450000100080000000500110000800800410001902200140006002400320007410000200010624501620012626000090028852013580029765000170165565000220167265300330169465300300172765300220175765300260177965300320180570000210183770000180185870000260187670000140190270000160191670000160193270000250194870000200197370000280199370000130202177300670203421509162023-12-20       2023    bl uuuu     u00u1 u    #d  a1365-26727 a10.1093/jambio/lxac0132DOI1 aSILVA, T. R. da  aNot just passengers, but co-pilots! Non-rhizobial nodule-associated bacteria promote cowpea growth and symbiosis with (brady)rhizobia.h[electronic resource]  c2023  aAims To isolate and characterize non-rhizobial nodule-associated bacteria (NAB) from cowpea root-nodules regarding their performance of plant-growth-promoting mechanisms and their ability to enhance cowpea growth and symbiosis when co-inoculated with bradyrhizobia. Methods and Results Sixteen NAB were isolated, identified, and in vitro evaluated for plant growth promotion traits. The ability to promote cowpea growth was analyzed when co-inoculated with Bradyrhizobium pachyrhizi BR 3262 in sterile and non-sterile substrates. The 16S rRNA gene sequences analysis revealed that NAB belonged to the genera Chryseobacterium (4), Bacillus (3), Microbacterium (3), Agrobacterium (1), Escherichia (1), Delftia (1), Pelomonas (1), Sphingomonas (1), and Staphylococcus (1). All strains produced different amounts of auxin siderophores and formed biofilms. Twelve out of the 16 strains carried the nifH, a gene associated with nitrogen fixation. Co-inoculation of NAB (ESA 424 and ESA 29) with Bradyrhizobium pachyrhizi BR 3262 significantly promoted cowpea growth, especially after simultaneous inoculation with the three strains. Conclusions NAB are efficient cowpea growth promoters and can improve the efficiency of the symbiosis between cowpea and the N-2-fixing microsymbiont B. pachyrhizi BR 3262, mainly under a specific triple microbial association.  aSiderophores  aVigna Unguiculata  aBiological nitrogen fixation  aBradyrhizobium pachyrhizi  aDiversity strains  aIindole 3 acetic acid  aNodules associated bacteria1 aRODRIGUES, R. T.1 aJOVINO, R. S.1 aCARVALHO, J. R. de S.1 aLEITE, J.1 aHOFMANN, A.1 aFISCHER, D.1 aRIBEIRO, P. R. de A.1 aROUWS, L. F. M.1 aFERNANDES JUNIOR, P. I.1 aRADL, V.  tJournal of Applied Microbiologygv. 134, n, 123, January 2023.