02105naa a2200277 a 450000100080000000500110000800800410001902400500006010000170011024501420012726000090026952012280027865000330150665000320153965000230157165000190159470000160161370000240162970000200165370000210167370000240169470000220171870000250174070000220176577300400178721508212023-01-09       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.3390/agronomy121231782DOI1 aARIYOSHI, C.  aDevelopment and validation of an allele-specific marker for resistance to bacterial halo blight in coffea arabica.h[electronic resource]  c2022  aBacterial halo blight (BHB) is a bacterial disease, caused by Pseudomonas syringae pv. garcae, which has been gaining prominence in the main coffee-producing regions. Chemical control of this disease increases production costs and is environmentally undesirable. In this scenario, the development of new cultivars resistant to BHB is the most economical and sustainable alternative. Marker-Assisted Selection (MAS) is an appropriate strategy to assist breeding programs for resistant genotype selection. In a previous Genome-Wide Association Study (GWAS) for C. arabica and P. syringae pv. garcae interaction, we identified a locus, probably linked to qualitative resistance to the pathogen. In this work, we developed and validated a pair of Allele-Specific-Polymerase Chain Reaction (AS-PCR) primers for this locus in C. arabica breeding populations. This pair of AS-PCR primers, called Psg_QL1, was tested both in a backcross (BC) (n = 38) and in an F2 population (n = 138) segregating for resistance to BHB. The linkage between the Psg_QL1 marker and qualitative resistance showed an accuracy of 93.75%. Our results demonstrated that the Psg_QL1 marker can be applied in MAS in a robust, simple, fast, and low-cost way.  aBacterial diseases of plants  aCoffea arabica var. arabica  aGenetic resistance  aPlant breeding1 aSERA, G. H.1 aRODRIGUES, L. M. R.1 aCARVALHO, F. G.1 aSHIGUEOKA, L. H.1 aMENDONÇA, A. E. S.1 aPEREIRA, C. T. M.1 aDESTÉFANO, S. A. L.1 aPEREIRA, L. F. P.  tAgronomygv. 12, n. 12, 3178, 2022.