03255nam a2200205 a 450000100080000000500110000800800410001910000260006024501590008626000150024530000110026050001780027152024810044965000190293065000250294965000190297465000120299365000190300565000250302421486592023-11-09       2022    bl uuuu  m   00u1 u    #d1 aROMERO PELÁEZ, R. D.  aInteractions between filamentous and yeast form fungi with biotechnological potential in the production of enzymes and metabolites.h[electronic resource]  a2022c2022  a156 f.  aThesis (Doctor in Microbial Biology) - University of Brasília, Brasília, DF.  Advisor: João Ricardo Moreira de Almeida;  Co-advisor: Félix Gonçalves de Siqueira, CNPAE.  aCo-cultures between microorganisms have shown important applications in various biotechnological processes, such as obtaining enzymes with high catalytic potential or prospecting for metabolites of commercial importance. This strategy is based on the molecular response to the interactions between two biological individuals, which may lead to increased enzymatic activities, secretion of bioactive compounds, or increased efficiency of plant biomass bioconversion. This thesis evaluates and describes the interactions with the filamentous fungi Panus lecomtei and Trichoderma reesei, and the yeast Sporidiobolus pararoseus as a function of the secretion of commercially important lignocellulolytic enzymes. The first stage of this study, addressed in Chapter I, involved the evaluation of the best conditions in the co-culture of P. lecomtei and T. reesei in submerged culture, where it was shown that temperature variations, the time between inocula, and medium composition characteristics define the production of an extract with high hydrolytic efficiency, as well as readily fermentable hydrolysates in ethanol. The proteomic analysis of the best condition for enzyme production in the co-culture of P. lecomtei and T. reesei, addressed in Chapter II, showed that the high hydrolysis efficiency might be related to the production of CAZy enzymes distributed in families with complete coverage of cellulases (endo-, exo- and betaglucosidases), in addition to various families associated with the degradation of hemicellulose and accessory enzymes such as lytic polysaccharide monooxygenases (LPMO). Finally, in Chapter III, it was shown that P. lecomtei responds positively in a coculture with S. pararoseus, increasing laccase production up to 2713.8 U/mL. This extract showed potential in the decolorization of RBBR industrial textile dye by about 80% and, even though this extract did not show significant cellulase activities. In addition, a cocktail with the extract from the best culture conditions of P. lecomtei and T. reesei allowed the highest percentage of hydrolytic efficiency among all conditions evaluated, with 75.69%, a novel result in the literature. This study demonstrates that the interactions between filamentous and yeast-like fungi, antagonistic or not, favor the generation of products with industrial potential. These may help in the future to solve problems related to the deconstruction of plant biomass or detoxification of polluted effluents.  aDecolorization  aEnzymatic hydrolysis  aLignocellulose  aCorante  aDescoloração  aEnzima Celulolítica