03073naa a2200445 a 450000100080000000500110000800800410001902200300006002400430009010000250013324501320015826000090029052017600029965000220205965000220208165000170210365000130212065000260213365000280215965000230218765000170221065000240222765000230225165300210227465300220229565300290231770000220234670000230236870000190239170000160241070000140242670000240244070000200246470000140248470000180249870000200251670000200253670000250255677300460258121486362022-11-28       2022    bl uuuu     u00u1 u    #d  a0031-9317 eISSN 1399-30547 ahttps://doi.org/10.1111/ppl.138212DOI1 aFERREIRA-NETO, R, C.  aDehydration response in Stylosanthesscabrabtranscriptional, biochemical, and physiological modulations.h[electronic resource]  c2022  atylosanthes scabra, popularly known as stylo, is native to the Brazilian Caatinga semi-arid region and stands out as a drought-tolerant shrub forage crop. This work pro-vides information about the plant response during the first 48 h of water deficit,followed by a rehydration treatment. Besides root transcriptomics data, 13 physiolog-ical or biochemical parameters were scrutinized. Additionally, RNA-Seq annotatedtranscripts not associated with the?Viridiplantae?clade were taxonomically catego-rized. It was found thatS. scabraquickly perceives and recovers from the oscillationsof the imposed water regime. Physiologically, mechanisms that minimize evapotrans-piration or protect the photosynthetic apparatus stood out. Biochemically, it wasfound that the root tissue invests in synthesizing compounds that can act as osmo-lytes (proline and sugars), emphasizing the importance of osmoregulation to waterdeficit acclimation. Consistently, transcriptome and qPCR analyses showed that a setof enriched biological processes with upregulated (UR) transcripts were involved inprotective functions against reactive oxygen species or encoding enzymes of impor-tant metabolic pathways, which might contribute toS. scabraresponse to water defi-cit. Additionally, several UR kinases and transcription factors were identified. Finally,in an innovative approach, some naturally occurring microbial groups (such asSchizo-saccharomyces,Bradyrhizobium, etc.) were identified in theS. scabraroots. This studyreveals insights into the physiological, biochemical, and molecular mechanisms under-lying theS. scabraresponse to water deficit and provides candidate genes that maybe useful in developing drought-tolerant crop varieties through biotechnologicalapplications  aAlternative crops  aDrought tolerance  aStylosanthes  aCaatinga  aDeficiência Hídrica  aDesidratação Química  aFisiologia Vegetal  aHidratação  aResistência a Seca  aVegetação Nativa  aDeficit hídrico  aForrageira nativa  aPlanta resistente a seca1 aARAÚJO, F. C. de1 aSILVA, R. L. de O.1 aMELO, N. F. de1 aPANDOLF, V.1 aFROSI, G.1 aMORAIS, D. A. de L.1 aSILVA, M. D. da1 aRIVAS, R.1 aSANTOS, M. G.1 aAIDAR, S. de T.1 aMORGANTE, C. V.1 aBENKO-ISEPPON, A. M.  tPhysiologia Plantarum, 174, e13821. 2022.