02593naa a2200421 a 450000100080000000500110000800800410001902400450006010000160010524501070012126000090022852014360023765000150167365000220168865000260171065000150173665000150175165000120176665000200177865000250179865300260182365300180184965300210186765300170188865300280190565300080193365300200194165300250196170000230198670000190200970000170202870000190204570000140206470000190207870000180209770000210211577300350213621485572022-12-01       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.3390/ani122231072DOI1 aFEDDERN, V.  aNicarbazin residue in tissues from broilers reared on reused litter conditions.h[electronic resource]  c2022  aAbstract: Nicarbazin (NCZ) is a worldwide used anticoccidial in poultry farming to avoid coccidiosis disease when chickens are reared on conventional poultry litter. If proper dosage and withdrawal time are not followed, the component dinitrocarbanilide (DNC) of NCZ may be present in chicken tissues, therefore posing a risk to consumers if the residues are above 200 ?g/kg. Litter reuse is a common and important practice in commercial chicken production. Literature is lacking about the influence of litter reuse on DNC deposition in chicken tissues and its impact on food safety. We aimed to evaluate DNC residues in breast and liver by LC?MS/MS from broilers from an experiment with 10 consecutive flocks during 2 years. The experiment included three treatments containing NCZ in the diet (T1 = 125 mg/kg, 1?21 d; T2 = 125 mg/kg, 1?32 d; T3 = 40 mg/kg, 1?32 d). DNC residues in chicken breast at 21 d in T1 ranged from 648.8?926 ?g/kg, at 32 d in T2 and T3 varied, respectively, from 232?667 ?g/kg and 52?189 ?g/kg. Regarding liver, DNC residues at 21 days in T1 ranged from 11,754?15,281 ?g/kg, at 32 days in T2 and T3 varied, respectively, from 10,168?15,021 ?g/kg and 2899?4573 ?g/kg. When NCZ was withdrawn from feed, DNC residues dropped to &ltLOQ at 42 d in all treatments. Therefore, the reuse of poultry litter does not compromise food safety regarding DNC residues in chicken tissues, as shown herein up to 10 flocks.  aAviculture  aChemical residues  aLiquid chromatography  aNicarbazin  aAvicultura  aFígado  aFrango de Corte  aSegurança Alimentar  aChicken breast fillet  aChicken liver  aDinitrocarbazina  aDNC residues  aFile de peito de frango  aNic  aPoultry farming  aResíduos de tecidos1 aSCHEUERMANN, G. N.1 aCOLDEBELLA, A.1 aGRESSLER, V.1 aBEDENDO, G. C.1 aCARON, L.1 aPEDROSO, A. C.1 aBACILA, D. M.1 aCUNHA JUNIOR, A.  tAnimalsgv. 12, n. 3107, 2022.