02407naa a2200385 a 450000100080000000500110000800800410001902200140006002400520007410000210012624501640014726000090031152012290032065000300154965000300157965000180160965000270162765000120165465000120166665000180167865000350169665000160173165000100174765000160175765300200177365300180179365300140181165300190182570000200184470000170186470000200188170000200190170000220192177300780194321464142022-09-14       2022    bl uuuu     u00u1 u    #d  a1878-81817 ahttps://doi.org/10.1016/j.bcab.2022.1024552DOI1 aLOCATELLI, G. O.  aProduction of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources.h[electronic resource]  c2022  aAbstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development.  aBiological control agents  aLiquid state fermentation  aSorghum flour  aTrichoderma asperellum  aCarbono  aFarinha  aFermentação  aFungo Para Controle Biológico  aNitrogênio  aSorgo  aTrichoderma  aAutolyzed yeast  aBiofungicides  aC N ratio  aMicrosclerotia1 aPIMENTEL, M. F.1 aBUENO, L. A.1 aLOBO JUNIOR, M.1 aMASCARIN, G. M.1 aFINKLER, C. L. L.  tBiocatalysis and Agricultural Biotechnologygv. 44, article 102455, 2022.