02585naa a2200349 a 450000100080000000500110000800800410001902200140006002400420007410000230011624501740013926000090031352015220032265000290184465000150187365000220188865000180191065000190192865000150194765000230196265000220198565000220200765000190202965000140204865300220206270000170208470000140210170000170211570000160213270000140214877300730216221458622022-08-30       2022    bl uuuu     u00u1 u    #d  a0951-41987 ahttps://doi.org/10.1002/rcm.93482DOI1 aSOUZA, E. C. A. de  aMolecular network-guided chemical profile and mass spectrometry, volatile compounds, and antimicrobial activity of Scaptotrigona depilis propolis.h[electronic resource]  c2022  aAbstract: Rationale Propolis has a great diversity in its composition due to numerous factors; therefore, each study is an important contribution to the knoFwledge of its composition and biological action. The objective of this study was to determine the chemical profile and biological activity of propolis produced by Scaptotrigona depilis. Methods Extracts with 70% ethanol (EPE70) and with cereal alcohol (CAPE) were elaborated, and then characterized using UHPLC-ESI(+)-MS/MS. Volatile compounds were extracted and then characterized using gas chromatography mass spectrometry (GC?MS). In addition, antimicrobial activities were verified against resistant strains. Results The volatile compounds of propolis predominantly consist of sesquiterpenes. Using the exploratory metabolomic approach, compounds of different classes were putatively identified in the ethanolic extracts, of which the most representative were terpenes, and some of the sesquiterpenes identified among the volatiles were also detected. The extracts were shown to be active against Escherichia coli and Staphylococcus aureus bacteria with a minimum inhibitory concentration (MIC) of 0.5 and 1.0 mg?mL?1, respectively. Conclusions The molecular network approach proved to be determining the chemical profile of S. depilis propolis rapidly and accurately, and led to the identification of lipophilic compounds. The identification of compounds using GC?MS and UHPLC-ESI(+)-MS/MS is complementary and useful for the characterization of propolis.  aAntibacterial properties  aHoney bees  aMass spectrometry  aScaptotrigona  aStingless bees  aTerpenoids  aVolatile compounds  aAbelha Brasileira  aAnálise Química  aEspectrometria  aPrópolis  aAbelha mandaguari1 aSILVA, B. P.1 aPORTO, C.1 aPILAU, E. J.1 aMENEZES, C.1 aFLACH, A.  tRapid Communications in Mass Spectrometry, V. 36, N. 19, e9348, 202.