01840naa a2200277 a 450000100080000000500110000800800410001902400530006010000170011324501320013026000090026230000100027152009880028165000130126965300250128265300220130765300090132965300190133870000220135770000210137970000170140070000220141770000230143970000260146277300740148821449022022-08-25       2022    bl uuuu     u00u1 u    #d7 attps://doi.org/10.1016/j.vetimm.2022.1104622DOI1 aOKINO, C. H.  aCD4 bovine genebdifferential polymorphisms among cattle breeds and a new tool for rapid identification.h[electronic resource]  c2022  a18 p.  aTwo mutations in the CD4 bovine gene (G&gtT/Q306H; A&gtC/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals.  aGenotype  aHigh Resolution Melt  aImmunophenotyping  aQPCR  aT helper cells1 aMALAGO JUNIOR, W.1 aMARCONDES, C. R.1 aGIGLIOTI, R.1 aMONTASSIER, H. J.1 aOLIVEIRA, H. N. DE1 aOLIVEIRA, M. C. de S.  tVeterinary Immunology and Immunopathologygv. 251, sep. 2022, 110462.