02066naa a2200241 a 450000100080000000500110000800800410001902400550006010000260011524501440014126000090028552012900029465000210158465000220160565300120162765300140163970000210165370000250167470000210169970000250172070000230174577300560176821395732022-08-08       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1080/10454438.2021.20165442DOI1 aBRASILIENSE, A. R. P.  aDifferent dietary protein levels for Podocnemis unifilis subadult farmingbhematological and biochemical assessment.h[electronic resource]  c2021  aThis study evaluated the physiological responses of subadult yellow-spotted Amazon river turtle, Podocnemis unifilis, to incremental crude protein levels (29%, 32%, 35%, and 39% CP) as extruded commercial feeds. The hematological and plasma bio-chemical parameters of P. unifilis were analyzed at 60, 120, and 180 days of the feeding trial. The increase in total thrombocyte, leukocyte, lymphocyte, heterophil, and eosinophil numbers, besides high respiratory burst activity showed an improvement in animal immune defense response to incremental protein in diets, acting together to maintain the integrity of the tissues against antigens and infectious agents. Elevated subadult P. unifilis plasma total cholesterol and triglyceride levels observed after increasing the feeding time (until 180 days) with incremented protein levels in artificial diets during captiv-ity are a nutritional warning, related to the condition of being less physically active. Based on these results, periodic physiological evaluations are particularly important to ensure the healthy and adequate nutritional conditions of captive-bred animals, such as maintaining lower stocking densities of animals to avoid stress and, if possible, providing them with fresh food in addition to extruded artificial feed.  aAnimal nutrition  aNutrição Animal  aGenetic  aGenotypes1 aMENDONÇA, R. P.1 aALMEIDA, P. E. M. de1 aDAMASCENO, L. F.1 aHOSHINO, M. D. F. G.1 aYOSHIOKA, E. T. O.  tJournal of Applied Aquaculture, p. 1-13, Dec. 2021.