02145naa a2200265 a 450000100080000000500110000800800410001910000190006024501150007926000090019452013700020365300290157365300230160265300190162565300150164465300200165970000160167970000250169570000190172070000250173970000170176470000220178170000170180377300590182021367752021-11-29       2019    bl uuuu     u00u1 u    #d1 aMAIA, D. S. V.  aListeria monocytogenes in sliced cheese and ham from retail markets in southern Brazil.h[electronic resource]  c2019  aABSTRACT The aims of this study were to evaluate the occurrence of Listeria monocytogenes and Salmonella spp. in sliced cheese and ham from retail markets in southern Brazil, as well as to perform molecular characterization and to assess the antimicrobial resistance profile of the isolates. Samples (n = 160) of sliced cheese and ham were collected at retail level from the city of Pelotas, Brazil. The isolation of L. monocytogenes and Salmonella spp. was performed and the isolates were confirmed by PCR, submitted to antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE). Listeria monocytogenes was found in 9.4% (15/160) of the samples. All L. monocytogenes isolates were positive for the prs, inlA, inlC and inlJ genes. Salmonella spp. was not isolated. Regarding the antimicrobial susceptibility, one (6.6%) L. monocytogenes isolate was resistant to streptomycin and four (26.6%) to clindamycin. Macrorestriction analysis with ApaI and AscI enzymes yielded two major PFGE groups I and II. All L. monocytogenes isolates showed virulence genes, and some of them were resistant to clinically used antimicrobials, representing a risk to public health. Moreover, PFGE patterns with high similarity were visualized in L. monocytogenes isolates at different times, demonstrating adaptability of the pathogen at retail level in the region.  aAntimicrobial resistance  aFoodborne pathogen  aPFGE RTE foods  aSerotyping  aVirulence genes1 aHAUBERT, L.1 aWÜRFEL, S. de F. R.1 aKRONING, I. S.1 aCARDOSO, M. R. de I.1 aLOPES, G. V.1 aFIORENTINI, A. M.1 aSILVA, W. P.  tFEMS Microbiology Lettersgv. 366, n. 22,p. 1-6, 2019.