02031naa a2200325 a 450000100080000000500110000800800410001902400520006010000210011224501240013326000090025752010860026665000140135265000120136665000150137865000120139365000160140565300230142165300140144465300250145865300250148365300210150865300240152965300220155370000230157570000230159870000220162170000180164377300440166121357062022-06-14       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s00709-021-01716-z2DOI1 aCHAVES, A. L. A.  aGenomic constitution, allopolyploidy, and evolutionary proposal for Cynodon Rich. based on GISH.h[electronic resource]  c2021  aPolyploidy is the main mechanism for chromosome number variation in Cynodon. Taxonomic boundaries are difcult to defne and, although phylogenetic studies indicate that some species are closely related, the degree of genomic similarity remains unknown. Furthermore, the Cynodon species classifcation as auto or allopolyploids is still controversial. Thus, this study aimed to investigate the genomic constitution in diploid and polyploid species using diferent approaches of genomic in situ hybridization (GISH). To better understand the hybridization events, we also investigated the occurrence of unreduced gametes in C. dactylon diploid pollen grains. We suggest a genomic nomenclature of diploid species as DD, D1 D1 , and D2 D2 for C. dactylon, C. incompletus, and C. nlemfuensis, and DDD2 D2 and DD2 D1 D1 for the segmental allotetraploids of Cynodon dactylon and C. transvaalensis, respectively. Furthermore, an evolutionary proposal was built based on our results and previous data from other studies, showing possible crosses that may have occurred between Cynodon species.  aCytotypes  aHybrids  aPolyploidy  aHibrido  aPoliploidia  aAnálise genômica  aCitótipo  aEvolutionary history  aGameta não reduzido  aGenomic analysis  aHistória evolutiva  aUnreduced gametes1 aCARVALHO, P. H. M.1 aFERREIRA, M. T. M.1 aBENITES, F. R. G.1 aTECHIO, V. H.  tProtoplasmagv. 259, p. 999-1011, 2022.