01852nam a2200205 a 450000100080000000500110000800800410001910000240006024500890008426001210017350000190029452012100031365000110152365000130153465000220154765000220156970000170159170000190160870000190162721348152023-04-25       2021    bl uuuu     u00u1 u    #d1 aANGELO, P. C. da S.  aDiversity in the regulatory region of genes in the SH3 locus.h[electronic resource]  aIn: ASIC CONFERENCE ON COFFEE SCIENCE, 28., 2021, Montpellier. Proceedings... Montpellier: ASIC, 2021. p. 227.c2021  aASIC. S2-P-20.  aRATIONALE: The SH3 locus is implicated in plant defense against the leaf rust disease caused by the fungi Hemileia vastatrix, which reduces coffee plant production. A cluster of genes coding for NB-LRR proteins is allocated to this locus. Investigating the regulatory region of' these genes can be worthy in knowledge and economically. METHODS: The cis-acting elements (cis-els, conserved motifs for the interaction with transcription machinery) in the promoters (2000 pb up-stream translation start) of 20 genes allocated to paralogous SH3 loci in four coffea spp. genotypes were identified and counted (www.dna.affrc.go.jp/place). Data were analyzed using Sigma Plot. RESULTS: Cis-els more frequently identified are depicted in Fig. 1 (in average, 3.5 times/promoter for SEF4MOTIFGM7S up to 14.1 times for CAATBOXl), Genes diverged for the frequency of cis-els. At least one gene/genotype was found in the highest cis-el frequency class (11.46 &lt N3, A2, Cl, B8 &gt 11.9 copies of each cis-el), probably displaying complex patters of induction. Genotypes did not diverge. CONCLUSIONS & PERSPECTIVES: Information presented here are going to be correlated with the transcription level of different genes.  aCoffea  aGenotype  aSpecies diversity  aGenética Vegetal1 aARIYOSHI, C.1 aCAIXETA, E. T.1 aPEREIRA, L. F.