02808naa a2200373 a 450000100080000000500110000800800410001902400560006010000200011624501480013626000090028452016750029365000130196865000170198165000120199865300180201065300280202865300170205665300190207370000280209270000180212070000250213870000220216370000200218570000190220570000230222470000260224770000220227370000170229570000170231270000190232970000200234877300660236821337852021-08-21       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.colsurfb.2020.1113712DOI1 aSILVA, N. P. da  aImproved anti-Cutibacterium acnes activity of tea tree oil-loaded chitosan-poly(?-caprolactone) core-shell nanocapsules.h[electronic resource]  c2020  aThe purpose of this study was to develop tea tree oil (TTO)-loaded chitosan-poly(?-caprolactone) core-shell nanocapsules (NC-TTO-Ch) aiming the topical acne treatment. TTO was analyzed by gas chromatographymass spectrometry, and nanocapsules were characterized regarding mean particle size (Z-average), polydispersity index (PdI), zeta potential (ZP), pH, entrapment efficiency (EE), morphology by Atomic Force Microscopy (AFM), and anti-Cutibacterium acnes activity. The main constituents of TTO were terpinen-4-ol (37.11 %), ?-terpinene (16.32 %), ?-terpinene (8.19 %), ?-cimene (6.56 %), and ?-terpineol (6.07 %). NC-TTO-Ch presented Z-average of 268.0 ± 3.8 nm and monodisperse size distribution (PdI &lt 0.3). After coating the nanocapsules with chitosan, we observed an inversion in ZP to a positive value (+31.0 ± 1.8 mV). This finding may indicate the presence of chitosan on the nanocapsules? surface, which was corroborated by the AFM images. In addition, NC-TTO-Ch showed a slightly acidic pH (~5.0), compatible with topical application. The EE, based on Terpinen-4-ol concentration, was approximately 95 %. This data suggests the nanocapsules? ability to reduce the TTO volatilization. Furthermore, NC-TTO-Ch showed significant anti-C. acnes activity, with a 4× reduction in the minimum inhibitory concentration, compared to TTO and a decrease in C. acnes cell viability, with an increase in the percentage of dead cells (17 %) compared to growth control (6.6 %) and TTO (9.7 %). Therefore, chitosan-poly(?-caprolactone) core-shell nanocapsules are a promising tool for TTO delivery, aiming at the activity against C. acnes for the topical acne treatment.  aChitosan  aTea tree oil  aÁrvore  aAcne vulgaris  aCore-shell nanocapsules  aNanocápsula  aQuitosana-poli1 aPEREIRA, E. do C. R. L.1 aDUARTE, L. M.1 aFREITAS, J. C. de O.1 aALMEIDA, C. G. de1 aSILVA, T. P. da1 aMELO, R. C. N.1 aAPOLONIO, A. C. M.1 aOLIVEIRA, M. A. L. de1 aBRANDAO, H. de M.1 aPITTELLA, F.1 aFABRI, R. L.1 aTAVARES, G. D.1 aPINTO, P. de F.  tColloids and Surfaces B: Biointerfacesgv. 196, 111371, 2020.