02333naa a2200325 a 450000100080000000500110000800800410001902400540006010000190011424501490013326000090028252013860029165000110167765000170168865000150170565000180172065000160173865300150175465300180176965300110178770000230179870000200182170000180184170000200185970000250187970000190190470000180192370000210194177300450196221337492024-04-15       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.livsci.2020.1043802DOI1 aMARSICO, T. V.  aCharacteristic MALDI-MS lipid profiles of Gir, Holstein and crossbred (Gir x Holstein) oocytes recovered by ovum pick-up.h[electronic resource]  c2021  aThe differential expression of many productive and reproductive traits has already been associated with the genotype effect observed between Bos taurus indicus (Bos indicus) and Bos taurus taurus (Bos taurus) breeds. Lipids have a crucial role during oocyte development. The aim of this work was to characterize the lipid profiles of oocytes recovered from dairy breeds: Gir (Bos indicus), Holstein (Bos taurus) and their crossbred (Gir x Holstein). Cumulus-oocyte complexes were collected by ovum pick-up from Gir (n=21), Holstein (n=36), and crossbreed (n=33) cows and submitted to MALDI-MS lipid fingerprint analysis. Multi- and univariate analyses were used to characterize lipid profiles of oocytes derived from each breed. Third lipid profiles (Gir n=7; Holstein n=12; and crossbred n=11) were evaluated and allowed characterization of oocyte?s lipids among breeds. The lipid profiles from Gir and Holstein were observed to be the most distinct, whereas Gir and crossbred oocytes presented greater similarities. Overall, a wide-range oocyte lipid profile characterization from distinctive bovine dairy breeds recovered by OPU were provided. We have identified two distinguishable lipid profile patterns among groups and revealed that phospholipids and triacylglycerol were the predominantly lipid assigned in Gir (Bos indicus) and Holstein (Bos taurus) oocytes, respectively.  aCattle  aDairy breeds  aMetabolism  aGado Leiteiro  aMetabolismo  aCrossbreed  aLipid profile  aOocyte1 aSALES, J. N. de S.1 aFERREIRA, C. R.1 aSUDANO, M. J.1 aVIANA, J. H. M.1 aCAMARGO, L. S. de A.1 aEBERLIN, M. N.1 aSENEDA, M. M.1 aBARUSELLI, P. S.  tLivestock Sciencegv. 243, 104380, 2021.