02496naa a2200397 a 450000100080000000500110000800800410001902400520006010000190011224501380013126000090026952013290027865000120160765000160161965000170163565000170165265000190166965000290168865000220171765300120173965300180175165300170176965300210178665300160180770000180182370000170184170000220185870000210188070000200190170000190192170000160194070000200195670000200197670000230199677300790201921335742021-12-07       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s42690-020-00275-92DOI1 aSARAIVA, N. B.  aMolecular profiling of the Mahanarva spectabilis salivary glands and phytohormonal response of elephant grass.h[electronic resource]  c2021  aSignal perception in plants is determinant to trigger specific physiological changes that confer resistance during the early stages of the insect attack. Thus, molecular characterization of both insect effectors and plant hormonal regulators are essential in developing forage grasses resistant to infestation by spittlebug Mahanarva spectabilis. We evaluated the presence of effectors in the salivary glands of M. spectabilis that could enable the infestation of elephant grass (Pennisetum purpureum). The presence of an NTPDase-like enzyme was confirmed, and higher activity was observed in females that hydrolyzed ADP. High levels of long chain fatty acids, such as octadecanoid acid (19.5%), prostaglandin A2 (4.7%), eicosanoid acid and the phytohormone salicylic acid (~4.0 μg/g) were also detected in the salivary glands. After 24 h of infestation, the phytohormone profiles were altered in leaves damaged by M. spectabilis, suggesting that the insect effectorsmodulate the plant response. Our results suggest that the plant responsemodulationmay be the result of crosstalk between the salicylic acid, jasmonic acid, abscisic acid and zeatin pathways, induced by molecules from the salivary glands. This shows that the spittlebugs can interfere with jasmonic acid and zeatin accumulation in elephant grass plants.  aApyrase  aFatty acids  aMetabolomics  aÁcido Graxo  aCapim Elefante  aCigarrinha das Pastagens  aHormônio Vegetal  aApirase  aFitohormônio  aPhytohormone  aPlant resistance  aSpittlebugs1 aEMIDIO, N. B.1 aVITAL, C. E.1 aGUSMÃO, M. A. N.1 aMARCONATO, D. G.1 aCOUTINHO, F. S.1 aPEREIRA, J. F.1 aAUAD, A. M.1 aFARIA-PINTO, P.1 aRAMOS, H. J. O.1 aOLIVEIRA, M. G. A.  tInternational Journal of Tropical Insect Sciencegv. 41, p. 849-860, 2021.