01797naa a2200277 a 450000100080000000500110000800800410001902400520006010000200011224501090013226000090024152009880025065000240123865000150126265000140127765000230129165300270131465300080134170000200134970000170136970000220138670000190140870000160142770000190144377300570146221334642021-08-11       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s11033-020-05445-z2DOI1 aLOPES, J. M. L.  aDevelopment of microsatellite markers for Lippia alba and related Lippia species.h[electronic resource]  c2020  aMicrosatellite primers were developed in Lippia alba complex to better understanding the origins and evolution of the species. We sought to increase the numbers of available simple sequence repeat (SSR) markers. We performed low-coverage (~ twofold) genomic DNA sequencing of a diploid accession and generated a de novo assembly comprising 175,572 contigs. Sixteen SSR loci were selected and of these 13 SSR loci were successfully amplified in 20 L. alba tetraploid accessions and in 12 other Lippia species. Only one SSR locus was monomorphic, whereas 12 loci were polymorphic, yielding one to nine alleles. The heterozygosity was similar among markers, with values of 0.274?0.485; the polymorphism information content values varied from 0.237 to 0.367. These markers were successfully amplified in related species with 85% of transferability on average. Thus, we demonstrate the utility of including a de novo assembly step to obtain SSR markers from low-coverage genomic datasets.  aPhyla (Verbenaceae)  aPolyploidy  aGenética  aMarcador Genético  aSimple sequence repeat  aSSR1 aMATOS, E. M. de1 aZORZATTO, C.1 aAZEVEDO, A. L. S.1 aMACHADO, M. A.1 aCHESTER, M.1 aVICCINI, L. F.  tMolecular Biology Reportsgv. 47, p. 4911-4915, 2020