02705naa a2200325 a 450000100080000000500110000800800410001902400520006010000160011224501560012826000090028450000870029352016900038065000160207065000130208665000110209965000110211065300240212165300130214570000200215870000170217870000200219570000220221570000200223770000180225770000190227570000200229470000210231477300440233521324012021-06-18       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1038/s41598-021-90767-62DOI1 aMELO, B. P.  aContrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses.h[electronic resource]  c2021  aNa publicação:  Isabela Tristan Lourenço-Tessutti; Maria Fátima Grossi-de-Sá.  aNACs are plant-specifc transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NACSAGs belong to well-characterized regulatory networks, poorly understood in soybean. Here, we interrogated the soybean genome and provided a comprehensive analysis of senescence-associated Glycine max (Gm) NACs. To functionally examine GmNAC-SAGs, we selected GmNAC065, a putative ortholog of Arabidopsis ANAC083/VNI2 SAG, and the cell death-promoting GmNAC085, an ANAC072 SAG putative ortholog, for analyses. Expression analysis of GmNAC065 and GmNAC085 in soybean demonstrated (i) these cell death-promoting GmNACs display contrasting expression changes during age- and stress-induced senescence; (ii) they are co-expressed with functionally diferent gene sets involved in stress and PCD, and (iii) are diferentially induced by PCD inducers. Furthermore, we demonstrated GmNAC065 expression delays senescence in Arabidopsis, a phenotype associated with enhanced oxidative performance under multiple stresses, higher chlorophyll, carotenoid and sugar contents, and lower stress-induced PCD compared to wild-type. In contrast, GmNAC085 accelerated stress-induced senescence, causing enhanced chlorophyll loss, ROS accumulation and cell death, decreased antioxidative system expression and activity. Accordingly, GmNAC065 and GmNAC085 targeted functionally contrasting sets of downstream AtSAGs, further indicating that GmNAC85 and GmNAC065 regulators function inversely in developmental and environmental PCD.  aArabidopsis  aSoybeans  aGenoma  aPlanta  aGmNAC065 expression  aNAC SAGs1 aLOURENCO, I. T.1 aFRAGA, O. T.1 aPINHEIRO, L. B.1 aLINS, C. B. de J.1 aMORGANTE, C. V.1 aENGLER, J. A.1 aREIS, P. A. B.1 aSA, M. F. G. de1 aFONTES, E. P. B.  tScientific Reportsgv. 11, 11178, 2021.