01546naa a2200361 a 450000100080000000500110000800800410001902000220006010000200008224000780010224500960018026000090027630000330028552003130031865000310063165000240066265000240068665000180071065000080072865000110073665000350074765300110078265300150079365300220080865300230083065300210085365300180087470000210089270000240091370000160093770000200095377302110097321320062021-05-28       2021    bl uuuu     u00u1 u    #d  a978-65-87380-10-01 aMORGANTE, C. V.  aModulação da expressão gênica em plantas via tecnologia CRISPR/dCas9.  aModulation of gene expression in plants via CRISPR/dCas9 technology.h[electronic resource]  c2021  aCAP. 4, 119-167cil., color.  aThis chapter discusses CRISPR technology using the dead Cas9 variant (CRISPR/ dCas9), emphasizing on its use for modulating gene expression in plants. The main strategies currently used are presented and discussed, for which a script was proposed, considering the main aspects for a good experimental design.  aAgricultural biotechnology  aGenetic engineering  aGenetic improvement  aBiotecnologia  aDNA  aGenoma  aMelhoramento Genético Vegetal  aCRISPR  aCRISPR Cas  aCRISPR technology  aExpressão gênica  aGenoma da planta  aSistema dCas91 aARRAES, F. B. M.1 aMOREIRA-PINTO, C. E1 aMELO, B. P.1 aSA, M. F. G. de  tIn: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021.