02172naa a2200217 a 450000100080000000500110000800800410001910000190006024501040007926000090018352015740019265000110176665000330177765000220181065000190183270000200185170000200187170000160189170000190190777300280192621298412021-02-09       2021    bl uuuu     u00u1 u    #d1 aLEASTRO, M. O.  aUnravelling the involvement of cilevirus p32 protein in the viral transport.h[electronic resource]  c2021  aCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread.  aCitrus  aPlant diseases and disorders  aDoença de Planta  aFruta Cítrica1 aASTUA, J. de F.1 aKITAJIMA, E. W.1 aPALLÁS, V.1 aNAVARRO, J. S.  tScientifc Reportsgn.11