01887naa a2200253 a 450000100080000000500110000800800410001902400510006010000220011124500960013326000090022952011250023865000140136365000130137765300270139065300210141770000300143870000260146870000200149470000220151470000190153670000170155577300610157221269112021-04-09       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.18512/rbms2020v19e11902DOI1 aLANA, U. G. de P.  aPolyphasic characterization of bacillus strains isolated from maize.h[electronic resource]  c2020  aThe use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subtilis (B2082 and B2084), using 16S rRNA gene sequencing, MALDI-TOF mass spectrometry and/or protein crystal detection. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are the same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants quality control, strains traceability and to detail PGPB colonization.  aBactéria  aZea Mays  aCrescimento de plantas  aPerfil molecular1 aRODRIGUES, M. E. G. de M.1 aOLIVEIRA-PAIVA, C. A.1 aSOUSA, S. M. de1 aTAVARES, A. N. G.1 aMARRIEL, I. E.1 aGOMES, E. A.  tRevista Brasileira de Milho e Sorgogv. 19, e1190, 2020.