02451naa a2200361 a 450000100080000000500110000800800410001902400280006010000210008824501320010926000090024152013860025065000170163665000100165365000210166365000220168465300190170670000260172570000230175170000190177470000150179370000200180870000180182870000250184670000190187170000170189070000250190770000140193270000220194670000210196870000190198977300810200821229792023-03-10       2020    bl uuuu     u00u1 u    #d7 a10.1111/tbed.135932DOI1 aROMANELLI, P. R.  aSeroepidemiology of ovine toxoplasmosis and neosporosis in breeding rams from Rio Grande do Sul, Brazil.h[electronic resource]  c2020  aThis study determined the prevalence of ovine toxoplasmosis and neosporosis and the risk factors associated with the development of these diseases in breeding rams from the State of Rio Grande do Sul (RS), Southern Brazil. Serum samples (n = 1,800) from breeding rams maintained on 705 sheep farms from seven mesoregions were evaluated serologically to detect anti-IgG Toxoplasma gondii by indirect ELISA and anti-IgG Neospora caninum by the indirect immunofluorescence assay (IFA). The prevalence of T. gondii was 33.05% (595/1,800); seropositivity to N. caninum was 18.44% (332/1,800). Additionally, there was simultaneous seropositivity (8.94%;161/1,800) to N. caninum and T. gondii. The variables size of the property (&lt500 ha) (Prevalence Ratio, PR = 1.36); breeding system (semi-intensive/intensive) (PR = 1.23); and natural mounting without control (PR = 1.50) were considered as risk factors for the occurrence of T. gondii. Size of the property (&lt500 ha) (PR = 1.58) and natural mounting without control (PR = 2.32) were risk factors associated with the prevalence of N. caninum in rams. Additionally, separation of ewes prior to parturition was considered as a protective factor for the occurrence of T. gondii (PR = 0.82) and N. caninum (PR = 0.74). These results demonstrated that these two parasitic disease agents are endemic in rams throughout all regions of RS.  aRisk factors  aSheep  aNeospora Caninum  aToxoplasma Gondii  aIndirect ELISA1 aMATOS, A. M. R. N. de1 aPINTO-FERREIRA, F.1 aCALDART, E. T.1 aMAREZE, M.1 aMATOS, R. L. N.1 aFREIRE, R. L.1 aMITSUKA-BREGANÓ, R.1 aHEADLEY, S. A.1 aMINHO, A. P.1 aCAVALCANTE, A. C. R.1 aOKANO, W.1 aKOHEK JÚNIOR, I.1 aRODRIGUES, R. O.1 aNAVARRO, I. T.  tTransboundary and Emerging Diseasesgv. 67, suppl. 2, p. 208-211, jul. 2020.