01538naa a2200241 a 450000100080000000500110000800800410001910000190006024501140007926000090019350001300020252006700033265000240100265000220102665300270104865300270107565300180110265300140112070000310113470000210116570000210118677300890120711122012023-03-30 2004 bl uuuu u00u1 u #d1 aCALIXTO, M. C. aSomatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck .h[electronic resource] c2004 aNotas Científicas. Título em português: Hibridação somática entre Citrus sinensis (L.) Osbeck e C. grandis (L.) Osbeck. aThis work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs. agenetic improvement aprotoplast fusion afusão de protoplastos amelhoramento genético aporta-enxerto arootstock1 aMOURÃO FILHO, F. de A. A.1 aMENDES, B. M. J.1 aVIEIRA, M. L. C. tPesquisa Agropecuária Brasileira, Brasília, DFgv. 39, n. 7, p. 721-724, jul. 2004