02959naa a2200361 a 450000100080000000500110000800800410001902400410006010000230010124501400012426000090026452018820027365000180215565000090217365000160218265000200219865000230221865000180224165000210225965300390228065300280231965300230234770000200237070000230239070000160241370000170242970000160244670000170246270000160247970000190249570000210251477300620253521213212020-03-19       2018    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1071/AN165672DOI1 aCOSTA, M. C. M. da  aEvaluation of internal and external markers to estimate faecal output and feed intake in sheep fed fresh forage.h[electronic resource]  c2018  aAbstract: The objectives of the present study were to estimate dry-matter intake and output of faeces using two external and four internal markers in sheep fed Brachiaria brizantha cv. Marandu on an ad libitum basis over 3- and 5-day periods. Six sheep fitted with ruminal cannulas were randomly assigned in a crossover design experiment to receive either of two treatments of external markers, namely titanium dioxide (TiO2) and chromic oxide (Cr2O3). Faecal output was obtained by total faecal collection and faecal grab sampling. Faeces were collected for 3- or 5-day periods, and, for each collection period, two sampling methods were compared; grab samples were collected directly in the rectum once daily, and a second sample was taken at the same time directly in faecal collection bags after having determined the daily total output of faeces. Faecal concentrations of the internal markers, indigestible dry matter, indigestible neutral detergent fibre, indigestible acid detergent fibre and indigestible acid detergent lignin (iADL), were determined. Faecal output was not accurately predicted with indigestible dry matter, indigestible neutral detergent fibre, indigestible acid detergent fibre and iADL. Dry-matter intake was predicted with iADL and TiO2 when faeces were collected for 5 days as grab samples once daily, or as total collection and with Cr2O3 when faecal grab samples were collected for 5 days. The results using external markers indicated that TiO2 is not a marker equivalent to Cr2O3 for estimating intake and faecal output. TiO2 was the only external marker to accurately estimate faecal output, independent of the method (total or grab) and time period (3 or 5 days) used; this suggests that TiO2 is the best marker tested for predicting the faecal output of sheep that are fed a diet of fresh Brachiaria brizantha (cv. Marandu) grass ad libitum.  aChromic oxide  aEwes  aFeed intake  aNutrient intake  aRuminant nutrition  aSheep feeding  aTitanium dioxide  aIndigestible acid detergent lignin  aIndigestible dry matter  aIndigestible fibre1 aITAVO, L. C. V.1 aITAVO, C. C. B. F.1 aDIAS, A. M.1 aPETIT, H. V.1 aREIS, F. A.1 aGOMES, R. C.1 aLEAL, E. S.1 aNIWA, M. V. G.1 aMORAES, G. J. de  tAnimal Production Sciencegv. 59, n. 4, p. 741-748, 2018.