02079naa a2200397 a 450000100080000000500110000800800410001910000200006024501010008026000090018152009850019065000100117565000150118565000110120065000190121165000200123065000120125065300110126265300240127365300130129765300220131065300100133270000190134270000200136170000210138170000240140270000310142670000230145770000240148070000340150470000200153870000250155870000190158370000230160277300560162521201712020-12-08       2019    bl uuuu     u00u1 u    #d1 aPINTO, T. M. F.  aEvaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus).  c2019  aAbstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed?s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization.  aGoats  aProteomics  aSaanen  aSemiarid zones  aSpermatogenesis  aCaprino  aBrasil  aBrazilian Northeast  aProfiles  aProteomic profile  aSperm1 aMOREIRA, R. F.1 aMATOS, M. N. C.1 aSOARES, V. V. M.1 aAGUIAR, M. V. de A.1 aARAGÃO, P. de T. T. D. de1 aALVES FILHO, J. G.1 aMORENO, F. B. M. B.1 aMONTEIRO-MOREIRA, A. C. de O.1 aCOSTA, C. R. R.1 aLIMA FILHO, J. L. de1 aELOY, A. M. X.1 aCUNHA, R. M. S. da  tAnimal Reproductiongv. 16, n. 4, p. 902-913, 2019.