01994naa a2200301 a 450000100080000000500110000800800410001910000180006024501540007826000090023252009770024165000240121865000280124265000390127065300930130970000180140270000170142070000210143770000230145870000190148170000210150070000170152170000220153870000170156070000180157770000200159577300770161521178632019-12-30       2019    bl uuuu     u00u1 u    #d1 aBACANELLI, G.  aMatrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae.h[electronic resource]  c2019  aIn this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.  aBovine tuberculosis  aMycobacterium bovis BCG  aMycobacterium tuberculosis complex  aMatrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry1 aOLARTE, L. C.1 aSILVA, M. R.1 aRODRIGUES, R. A.1 aCARNEIRO, P. A. M.1 aKANEENE, J. B.1 aPASQUATTI, T. N.1 aTAKATANI, H.1 aZUMÁRRAGA, M. J.1 aETGES, R. N.1 aARAUJO, F. R.1 aVERBISCK, N. V.  tJournal the Veterinary Medical Sciencegv. 81, n. 10, p.1400?1408, 2019.