02597naa a2200325 a 450000100080000000500110000800800410001902400520006010000210011224502060013326000090033952015510034865000150189965000310191465000140194565000100195965000140196965300260198365300210200965300180203070000200204870000170206870000160208570000200210170000270212170000190214870000240216770000150219177300650220621133882023-03-13       2019    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s10493-019-00368-22DOI1 aPOLITI, F. A. S.  aEvaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (AcaribIxodidae).h[electronic resource]  c2019  aThe cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (TpEtOH) showed an efcacy of 99.2% (LC50=18.60 mg mL−1). From the repellency test, it was found that both the TpEtOH and essential oil were 100% efcient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with TpEtOH (LC50=3.798 mg mL−1). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent efect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50&gt4000 mg kg−1), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to help control populations of ticks in the feld.  aAcaricides  aAnimal parasites and pests  aLivestock  aTicks  aCarrapato  aEctoparasitic control  aNatural products  aSpotted fever1 aFANTATTO, R. R.1 aSILVA, A. da1 aMORO, I. J.1 aSAMPIERI, B. R.1 aCAMARGO-MATHIAS, M. I.1 aFIGUEIREDO, A.1 aCHAGAS, A. C. de S.1 aFURLAN, M.  tExperimental and Applied Acarologygv. 77, p. 601-618, 2019.