01277naa a2200277 a 450000100080000000500110000800800410001902400350006010000200009524501410011526000090025652004370026565000150070265000120071765000200072965000220074965300170077165300260078865300290081465300160084365300270085965300140088670000190090070000200091977300600093921125562019-09-27 2006 bl uuuu u00u1 u #d7 a10.1007/s11240-006-9121-32DOI1 aTEIXEIRA, S. L. aInfluence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior.h[electronic resource] c2006 aA new protocol for the preparation and sterilization of media with sodium hypochlorite was tested for in vitro culturing of pineapple shoots. Total active chlorine concentrations equal to or higher than 0.0003%, as well as other asepsis procedures, provided complete sterilization of the medium. At a total active chlorine concentration of 0.0003%, pineapple shoots more than doubled both their biomass and the number of new shoots. aPineapples aAbacaxi aAnanás Comosus aMicropropagação aAutoclavagem aContaminação média aEsterilização química aHipoclorito aProdução de biomassa aProtocolo1 aRIBEIRO, J. M.1 aTEIXEIRA, M. T. tPlant Cell Tiss Organ Culturegv. 86, p. 375?337, 2006.