02040naa a2200289 a 450000100080000000500110000800800410001902400350006010000170009524501800011226000090029252011430030165000180144465000110146265000150147365000210148865000140150965000110152365300210153465300210155570000230157670000190159970000160161870000200163470000180165477300780167221110982019-09-24       2016    bl uuuu     u00u1 u    #d7 a10.1007/s12010-015-1955-x2DOI1 aMICHELON, W.  aEffects of nitrogen and phosphorus on biochemical composition of microalgae polyculture harvested from phycoremediation of piggery wastewater digestate.h[electronic resource]  c2016  aAbstract: The effects of nitrogen (N) and/or phosphorus (P) starvation on the biochemical composition of native microalgae Chlorella spp. polyculture obtained from the phycoremediation of swine wastewaters were investigated. Microalgae-specific growth rate of 1.2 day(-1) was achieved (30.3 mg L(-1) day(-1)). PO4 (-2) and NH3 were completely removed from swine digestate effluent after 3 and 11 days, respectively. Microalgae harvested immediately after nutrient removal showed high protein (56-59 %) and carbohydrate (25-34 %) but low lipid (1.8-3 %) contents. Depletion of N or P alone stimulated carbohydrate production at the expenses of proteins. Significant lipid accumulation from 3 % ± 0.5 to 16.3 % ± 0.8 was reached only after 25 days following N and P starvation as demonstrated by Nile red-stained cells. Regarding to the effects of harvesting methods on cellular biochemical composition, circumstantial evidences indicate that coagulation-flocculation with tannin may lead to lower protein and lipid amounts but increased carbohydrate content (p &lt 0.01) as compared to centrifugation.  aCarbohydrates  aLipids  aMicroalgae  aÁguas Residuais  aMicroalga  aSuíno  aPhycoremediation  aSwine wastewater1 aSILVA, M. L. B. da1 aMEZZARI, M. P.1 aPIROLLI, M.1 aPRANDINI, J. M.1 aSOARES, H. M.  tApplied Biochemistry and Biotechnologygv. 178, n. 7, p. 1407-1419, 2016.