02626naa a2200409 a 450000100080000000500110000800800410001902400350006010000180009524501290011326000090024230000090025152014170026065000190167765000280169665000200172465000210174465000200176565000270178565000150181265000150182765000190184265300330186165300180189465300170191265300240192965300310195365300370198465300240202170000180204570000180206370000220208170000180210370000170212170000210213877300570215921089062020-02-17       2019    bl uuuu     u00u1 u    #d7 a10.1186/s12917-019-1843-72DOI1 aSOARES, S. M.  aIn vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus.h[electronic resource]  c2019  a9 p.  aBackground: Haemonchosis affects sheep husbandry and its treatment is often compromised due to the development of anthelminthic resistance. Plant-derived bioactive compounds have been studied as alternative to control Haemonchus contortus. The objective of this study was to evaluate the effect of Senecio brasiliensis extracts on H. contortus egg hatching and infective larvae migration. Results: The aqueous extract from dried and fresh plant and alkaloid-enriched fraction of the previously dried leaves of S. brasiliensis inhibited H. contortus egg hatching. The main plant compound in alkaloid fraction was integerrimine, a pyrrolizidine alkaloid (PA). However, the aqueous extract from dried plant displayed higher efficacy when compared to their alkaloid enriched or non-polar fractions, meaning that, although PAs contributed to the ovicidal effect, other compounds in the plant can also contribute to their effect. Furthermore, the aqueous extract from dried plant also had higher efficacy than aqueous extract from fresh plant in larvae migration inhibition. Finally, extract from dried plant presented low in vitro cytotoxic effect. Conclusion: Taken together our results suggest a good anthelmintic effect of S. brasiliensis, especially when aqueous extract is prepared from dried plant. Further in vivo studies should be performed focused on forms of administration of this extract in rearing sheep.  aPlant extracts  aPyrrolizidine alkaloids  aSmall ruminants  aAnti-Helmíntico  aExtrato Vegetal  aIncubação Artificial  aMaria Mole  aNematóide  aPlanta Tóxica  aAlcalóides de pirrolizidina  aAntelmíntico  aEgg hatching  aIncubação de ovos  aInfective larvae migration  aMigração de larvas infectantes  aPequenos ruminantes1 aDOMINGUES, R.1 aGASPAR, E. B.1 aSANTOS, P. A. dos1 aCANUTO, K. M.1 aMINHO, A. P.1 aVIEIRA, M. I. B.  tBMC Veterinary Researchgv. 15, n. 99, 25 Mar. 2019.