02289naa a2200277 a 450000100080000000500110000800800410001910000260006024502020008626000090028852014010029765300220169865300140172065300140173465300250174870000240177370000200179770000200181770000210183770000200185870000170187870000190189570000180191470000160193277300630194821071202019-11-18       2019    bl uuuu     u00u1 u    #d1 aMESQUITA, L. M. de S.  aLC-ESI-IT-MS/MS and MALDI-TOF approachbidentification of natural polymers from rhizophora mangle barks and determination of their analgesic and anti-inflammatory properties.h[electronic resource]  c2019  aWe recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)?ESI?IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]?FIA?IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 ? 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of - 16 Da (homologous series of afzelechins?m/z 873?3465 Da) and ? 16 Da (homologous series of gallocatechins?m/z 905?3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779?3371) and glucoafzelechins (m/z 747?3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 lg mL-1) have a morphine-like effect and intense anti-inflammatory activity.  aAnti-inflammatory  aCatechins  aMangroves  aMorphine-like effect1 aRODRIGUES, C. F. B.1 aROCHA, C. Q. da1 aBIANCHIM, M. S.1 aRODRIGUES, C. M.1 aOLIVEIRA, V. de1 aGAETA, H. H.1 aBELCHOR, M. N.1 aTOYAMA. M. H.1 aVILEGAS, W.  tNatural Products and Bioprospectinggv. 9, p. 23-34, 2019.