02585naa a2200373 a 450000100080000000500110000800800410001902400340006010000200009424501420011426000090025652015090026565000160177465000170179065000220180765000180182965000190184765300170186665300270188365300190191065300150192970000160194470000130196070000170197370000200199070000200201070000180203070000150204870000230206370000190208670000230210570000260212877300570215421071172019-12-02       2019    bl uuuu     u00u1 u    #d7 a10.3389/fgene.2019.002102DOI1 aDINIZ, W. J. S.  aDetection of Co-expressed pathway modules associated with mineral concentration and meat quality in nelore cattle.h[electronic resource]  c2019  aMeat quality is a complex trait that is influenced by genetic and environmental factors, which includes mineral concentration. However, the association between mineral concentration and meat quality, and the specific molecular pathways underlying this association, are not well explored. We therefore analyzed gene expression as measured with RNA-seq in Longissimus thoracis muscle of 194 Nelore steers for association with three meat quality traits (intramuscular fat, meat pH, and tenderness) and the concentration of 13 minerals (Ca, Cr, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, and Zn). We identified seven sets of co-expressed genes (modules) associated with at least two traits, which indicates that common pathways influence these traits. From pathway analysis of module hub genes, we further found an over-representation for energy and protein metabolism (AMPK and mTOR signaling pathways) in addition to muscle growth, and protein turnover pathways. Among the identified hub genes FASN, ELOV5, and PDE3B are involved with lipid metabolism and were affected by previously identified eQTLs associated to fat deposition. The reported hub genes and over-represented pathways provide evidence of interplay among gene expression, mineral concentration, and meat quality traits. Future studies investigating the effect of different levels of mineral supplementation in the gene expression and meat quality traits could help us to elucidate the regulatory mechanism by which the genes/pathways are affected.  aBeef cattle  aBeef quality  aIntramuscular fat  aGado de Corte  aGordura Animal  aAMPK pathway  aCo-expression analysis  aRNA sequencing  aTenderness1 aMAZZONI, G.1 aCOUTINHO1 aBANERJEE, P.1 aGEISTLINGER, L.1 aCESAR, A. S. M.1 aBERTOLINI. F.1 aAFONSO, J.1 aOLIVEIRA, P. S. N.1 aTIZIOTO, P. C.1 aKADARMIDEEN, H. N.1 aREGITANO, L. C. de A.  tFrontiers in geneticsgv. 10, n. 210, p. 1-12, 2019.