01845naa a2200277 a 450000100080000000500110000800800410001910000150006024501470007526000090022252009860023165000230121765000260124065000230126665300220128970000260131170000260133770000190136370000200138270000200140270000170142270000230143970000170146270000210147977300670150021061852019-02-18       2019    bl uuuu     u00u1 u    #d1 aDIB, H. X.  aBiochemical characterization of a Kunitz inhibitor from Inga edulis seeds with antifungal activity against Candida spp.h[electronic resource]  c2019  aWe describe the characterization of IETI, the first trypsin inhibitor purified from Inga edulis, a tree widely distributed in Brazil. Two-step chromatography was used to purify IETI, a protein composed of a single peptide chain of 19,685.10 Da. Amino-terminal sequencing revealed that IETI shows homology with the Kunitz family, as substantiated by its physical?chemical features, such as its thermal (up to 70 °C) and wide-range pH stability (from 2 to 10), and the value of its dissociation constant (6.2 nM). IETI contains a single reactive site for trypsin, maintained by a disulfide bridge; in the presence of DTT, its inhibitory activity was reduced in a time- and concentration-dependent manner. IETI presented activity against Candida ssp., including C. buinensis and C. tropicalis. IETI inhibitory activity triggered yeast membrane permeability, affecting cell viability, thus providing support for the use of IETI in further studies for the control of fungal infections.  aCandida tropicalis  aMembrane permeability  aTrypsin inhibitors  aCandida buinensis1 aOLIVEIRA, D. G. L. de1 aOLIVEIRA, C. F. R. de1 aTAVEIRA, G. B.1 aMELLO, E. de O.1 aVERBISCK, N. V.1 aCHANG, M. R.1 aCORRÊA JUNIOR, D.1 aGOMES, V. M.1 aMACEDO, M. L. R.  tArchives of Microbiologygv. 201, n. 2, p. 223?233, March 2019