02332naa a2200433 a 450000100080000000500110000800800410001910000130006024500840007326000090015750001490016652010460031565000260136165000110138765000110139865000120140965000180142165000140143965000130145365000200146665000110148665000130149765000170151065000190152765000160154665300280156265300200159065300170161065300320162765300130165970000160167270000180168870000170170670000190172370000200174270000210176270000180178377300970180121040732019-01-14 2018 bl uuuu u00u1 u #d1 aBIER, D. aSurvey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil. c2018 aTítulo em português: Pesquisa de Salmonella spp. em carcaças bovinas durante o processamento em abatedouros‑frigoríficos exportadores. aThe aim of the present study was to investigate the presence of Salmonella spp. in samples collected from beef meat at three points of the slaughter line (after skinning, washing and cooling) at three slaughterhouses in Brazil that export meat. Detection was based on ISO 6579:2002 and confirmed by PCR and qPCR. The isolates were typified using slide agglutination tests and PFGE. The antibiotic sensitivity profile was determined using the disk diffusion method. Contamination was detected in only one slaughterhouse. The overall frequency of contamination by Salmonella spp. was 6.7% of carcasses (6/90) and 2.6% of carcass surface samples (7/270). All isolates were confirmed by PCR and qPCR. The serological analysis and the PFGE showed a single profile: Typhimurium. The strains demonstrated 100% susceptibility to ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin and tetracycline. Positive carcasses after cooling pose a direct risk to consumers, since the meat is considered ready to be marketed after this process. aAntibiotic resistance aBrazil aCattle aCooling aSalmonellosis aSerotypes aSkinning aSlaughterhouses aBovino aCarcaça aResfriamento aSalmonella spp aSalmonelose aAbatedouro-frigorífico aCarcaça bovina aCarne bovina aResistência a antibiótico aSorotipo1 aKICH, J. D.1 aDUARTE, S. C.1 aSILVA, M. R.1 aVALSONI, L. M.1 aRAMOS, C. A. N.1 aRODRIGUES, D. P.1 aARAUJO, F. R. tPesquisa Veterinária Brasileira, Rio de Janeirogv. 38, n. 11, p. 2037-2043, novembro, 2018