02870naa a2200337 a 450000100080000000500110000800800410001902400270006010000190008724501380010626000090024450000850025352018360033865000220217465000220219665300160221865300200223465300310225465300300228570000190231570000230233470000150235770000150237270000120238770000220239970000210242170000160244270000130245870000200247177300410249121029422019-04-27       2018    bl uuuu     u00u1 u    #d7 a10.1111/ppa.129702DOI1 aVALENTE, J. B.  aA novel putative member of the family Benyviridae is associated with soilborne wheat mosaic disease in Brazil.h[electronic resource]  c2018  aDIsponível em: &lthttps://onlinelibrary.wiley.com/doi/epdf/10.1111/ppa.12970&gt  aSoilborne wheat mosaic disease (SBWMD), originally attributed to infections by Soilborne wheat mosaic virus (SBWMV) and Wheat spindle streak mosaic virus (WSSMV), is one of the most frequent virus diseases and causes economic losses in wheat in southern Brazil. This study aimed to characterize molecularly the viral species associated with wheat plants showing mosaic symptoms in Brazil. Wheat leaves and stems displaying mosaic symptoms were collected from different wheat cultivars in Passo Fundo municipality, Rio Grande do Sul State, southern Brazil. Double-stranded RNA was extracted and submitted to cDNA library synthesis and next-generation sequencing. No sequences of SBWMV and WSSMV were detected but the complete genome sequence of a putative new member of the family Benyviridae was determined, for which the name wheat stripe mosaic virus (WhSMV) is proposed. WhSMV has a bipartite genome with RNA 1 and RNA 2 organization similar to that of viruses belonging to Benyviridae . WhSMV RNA 1 has a single open reading frame (ORF) encoding a polyprotein with putative viral replicase function. WhSMV RNA 2 has six ORFs encoding the coat protein, the major protein (read-through), triple gene block movement proteins (TGB 1, 2 and 3) and ORF 6 (hypothetical protein). In addition to the genomic organization and nucleotide and amino acid sequence identi- ties, phylogenetic analyses also corroborated that WhSMV is a virus species of the Benyviridae . However, isolates of WhSMV formed a clade distinct from members of the genus Benyvirus . It was also demonstrated that the plasmodio- phorid Polymyxa graminis is associated with wheat roots showing SBWMD symptoms and infected by WhSMV. Keywords: Benyviridae , mosaic symptoms, next-generation sequencing, Polymyxa graminis , Triticum aestivum , wheat stripe mosaic virus  aPolymyxa Graminis  aTriticum Aestivum  aBenyviridae  aMosaic symptoms  aNext-generation sequencing  aWheat stripe mosaic virus1 aPEREIRA, F. S.1 aSTEMPKOWSKI, L. A.1 aFARIAS, M.1 aKUHNEM, P.1 aLAU, D.1 aFAJARDO, T. V. M.1 aNHANI JUNIOR, A.1 aCASA, R. T.1 aBOGO, A.1 aSILVA, F. N. da  tPlant Pathology, First online, 2018.