02151naa a2200361 a 450000100080000000500110000800800410001902200140006002400360007410000220011024501770013226000090030952010330031865000100135165000350136165000130139665000430140965000250145265000120147765000270148965000230151665000150153965300330155465300220158770000200160970000140162970000170164370000180166070000200167870000200169870000200171877300510173821005572019-01-22       2018    bl uuuu     u00u1 u    #d  a2212-42927 a10.1016/j.fbio.2018.10.0072DOI1 aVENTURELLI, G. L.  aNew plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR.h[electronic resource]  c2018  aThe geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These plasmids are suitable to be used as CRM for Embrapa 5.1 GM common bean. They will be useful for survey of food labels for compliance with legislation about GMO content in Brazil and in other countries where GM common bean is not yet approved for commercialization.  aBeans  aGenetically modified organisms  aPlasmids  aQuantitative polymerase chain reaction  aEngenharia Genética  aFeijão  aOrganismo Transgênico  aPhaseolus Vulgaris  aPlasmídeo  aCertified reference material  aEMB-PV051-1-event1 aSILVA, K. J. da1 aTREML, D.1 aNAVAS, P. B.1 aVARGAS, M. O.1 aBISCHOFF, J. L.1 aFARIA, J. C. de1 aARISI, A. C. M.  tFood Biosciencegv. 26, p. 145-151, Dec. 2018.