01445naa a2200229 a 450000100080000000500110000800800410001910000190006024501040007926000090018330000130019252006790020565000180088465000120090265300230091465300150093765300190095270000190097170000210099070000230101177301810103421003592019-06-25       2019    bl uuuu     u00u1 u    #d1 aMARTINS, P. K.  aSetaria viridis as a model plant for functional genomic studies in C4 Crops.h[electronic resource]  c2019  ap. 49-64  aSetaria viridis is an emerging model for C4 species, and it is an important model to validate some genes for further C4 crop transformation, such as sugarcane, maize, and wheat. Here, we describe two protocols for stable transformation of S. viridis mediated by Agrobacterium tumefaciens with three different reporter genes and two selectable markers. Routine transformation efficiency reaching 29% was achieved using embryogenic callus in S. viridis (accession A10.1). Alternatively, we developed a transformation method by floral dip with 0.6% efficiency. The developed protocols could be useful for genetic and genomics studies of important food-feed-fiber-fuel C4 crops.  aAgrobacterium  aGrasses  aEmbryogenic callus  aFloral dip  aTransformation1 aDIAS, B. B. A.1 aKOBAYASHI, A. K.1 aMOLINARI, H. B. C.  tIn: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biologygv. 1864).