02427naa a2200337 a 450000100080000000500110000800800410001902400520006010000160011224501570012826000090028552013770029465000150167165000210168665000210170765000260172865000180175465000140177265000090178665300200179565300340181565300350184965300290188470000270191370000230194070000140196370000170197770000200199470000240201477300510203820989812021-11-11       2018    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1038/s41598-018-33594-62DOI1 aCUER, C. A.  aShort-term effect of Eucalyptus plantations on soil microbial communities and soil-atmosphere methane and nitrous oxide exchange.h[electronic resource]  c2018  aSoil greenhouse gas (GHG) emissions are a significant environmental problem resulting from microbially-mediated nitrogen (N) and carbon (C) cycling. This study aimed to investigate the impact of Eucalyptus plantations on the structure and function of a soil microbial community, and how resulting alterations may be linked to GHG fluxes. We sampled and monitored two adjacent Eucalyptus plantations - a recently logged site that harbored new seedlings and an adult plantation - and compared them to a site hosting native vegetation. We used 16S rRNA gene sequencing and qPCR amplifications of key nitrogen and methane cycle genes to characterize microbial structure and functional gene abundance and compared our data with soil parameters and GHG fluxes. Both microbial community attributes were significantly affected by land use and logging of Eucalyptus plantations. The genes nosZ and archaeal amoA were significantly more abundant in native forest than in either young or old Eucalyptus plantations. Statistical analyses suggest that land use type has a greater impact on microbial community structure and functional gene abundance than Eucalyptus rotation. There was no correlation between GHG fluxes and shifts in microbial community, suggesting that microbial community structure and functional gene abundance are not the main drivers of GHG fluxes in this system.  aEucalyptus  aGreenhouse gases  aGreenhouse soils  aMicrobial communities  aEfeito Estufa  aEucalipto  aGás  aAtlantic Forest  aEmissions of greenhouse gases  aGases de efeito estufa do solo  aSoil microbial processes1 aRODRIGUES, R. de A. R.1 aBALIEIRO, F. de C.1 aJESUS, J.1 aSILVA, E. P.1 aALVES, B. J. R.1 aRACHID, C. T. C. C.  tScientific Reportsgv. 8, article 15133, 2018.