01935naa a2200193 a 450000100080000000500110000800800410001902200140006010000170007424501850009126000090027652013140028565000120159965000130161170000190162470000200164370000260166377300520168920960592018-09-28       2018    bl uuuu     u00u1 u    #d  a1983-20521 aSILVA, L. L.  aDifferentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP.h[electronic resource]  c2018  aDifferent species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing.  aCassava  aMandioca1 aPESTANA, K. N.1 aFERREIRA, C. F.1 aOLIVEIRA, S. A. S. de  tTropical Plant Pathologygv.43, p.194-20, 2018.